In the era of super-resolved fluorescence microscopy [1], one of the fundamental aspects is related
to the relationship between the biophysical question and the specimen preparation [2] to access
the nanoscale in terms of spatial and temporal resolution when using a targeted [3] or a stochastic
approach [4]. Minflux [5] is a comparatively new approach towards imaging and tracking with a
perspective of localizing single fluorescent molecules with precision at the Angstrom level [6]. This
project focuses on those aspects related to specimen preparation to get microscopical and
spectroscopical quantitative information when using super-resolved fluorescence architectures.
[1] Diaspro, A., Bianchini, P. Optical nanoscopy. Riv. Nuovo Cim. 43, 385–455 (2020).
[2] M. Cosentino, C. Canale, P. Bianchini, A. Diaspro, Sci. Adv. 5, eaav8062 (2019).
[3] G. Vicidomini, P. Bianchini, A. Diaspro, Nat. Methods 15, 173 (2018)
[4] H. Deschout, F.C. Zanacchi, M. Mlodzianoski, A. Diaspro, J. Bewersdorf, S.T. Hess, K. Braeckmans,
Nat. Methods 11, 253 (2014)
[5] F. Balzarotti, Y. Eilers, K.C. Gwosch, A.H. Gynnå, V. Westphal, F.D. Stefani, J. Elf, S.W. Hell, Science
355, 606 (2017).
[6] Weber, M., von der Emde, H., Leutenegger, M. et al. MINSTED nanoscopy enters the Ångström
localization range. Nat Biotechnol 41, 569–576 (2023).
Docenti di riferimento
Stefania Abbruzzetti stefania.abbruzzetti@unipr.it
Alberto Diaspro diaspro@fisica.unige.it
